siMMP-2-treated mice showed increased elastin-to-collagen ratio, lower plasma desmosine (DES), enhanced phosphorylation of endothelial nitric oxide synthase (eNOS), and higher levels of vascular cyclic guanosine monophosphate (cGMP). MMP-2 knockdown reduced vascular MMP-2 levels and attenuated age-dependent carotid stiffness. Carotid PWV was assessed at baseline, 2 and 4 weeks after start of the treatment. Old WT mice (18- to 21-month old) were treated for 4 weeks with either MMP-2 or scrambled (Scr) siRNA via tail vein injection. Carotid PWV as well as vascular and circulating MMP-2 were elevated with increasing age in mice. MMP-2 levels in the carotid artery and plasma of young (3 months) and old (20-25 months) WT mice were determined. Pulse wave velocity (PWV) was assessed in right carotid artery of wild-type (WT) mice from different age groups. Thus, we aimed to investigate the efficacy of MMP-2 knockdown using small-interfering RNA (siRNA) on age-dependent arterial stiffness. ![]() ![]() However, the mechanistic link between age-dependent arterial stiffness and MMP-2 remains unclear. Age-dependent stiffening of large elastic arteries is primarily attributed to increased levels of matrix metalloproteinase-2 (MMP-2).
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